Ruscombe Brook is a small river in Gloucestershire, England. It rises on the southern edge of the village of Ruscombe and flows southwards. At Puckshole it is joined on its right bank by a tributary originating near Randwick. It continues southwards and flows into the Stroudwater Navigation, although formerly it passed under the canal in a culvert and flowed into the River Frome. Historically, it supplied power to several water mills.